NAD+

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Purity Guarantee

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every cell of the body, functioning as a required cofactor in mitochondrial energy production, DNA repair, and sirtuin activation. NAD+ levels decline approximately 40–50% between ages 20 and 60, and this age-related NAD+ decline is a central focus of longevity and mitochondrial research. Oral NAD+ precursors NMN (nicotinamide mononucleotide) and NR (nicotinamide riboside) raise intracellular NAD+ levels, but conversion efficiency varies between individuals and is subject to first-pass gut and hepatic metabolism. Injectable NAD+ delivers the intact coenzyme directly into systemic circulation, bypassing gut conversion and the bioavailability limits of precursor supplementation.

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Purity guarantee. If an independent assay of your lot returns below the purity stated on its certificate, we replace the order or refund it in full — no return shipment required.

Sold for laboratory research and further manufacturing use only. Not a drug, food, cosmetic, or dietary supplement. Not for human or veterinary use, and not for diagnostic or therapeutic purposes. Buyer is responsible for compliance with all applicable regulations.

Lyophilized Research Compound

Technical Overview

Nicotinamide adenine dinucleotide (NAD⁺) is a central redox cofactor involved in a wide range of cellular metabolic reactions, cycling between its oxidized (NAD⁺) and reduced (NADH) forms to shuttle electrons through dehydrogenase-dependent pathways.

In laboratory settings, NAD⁺ is widely used as a biochemical reagent for enzymatic assays, redox pathway analysis, and metabolic pathway evaluation. Common applications include dehydrogenase activity assays, NAD⁺/NADH ratio measurement, cellular respiration and glycolysis studies, and sirtuin and PARP enzyme research, where NAD⁺ serves as the required substrate.

This NAD⁺ reagent is prepared to analytical research standards to support consistent, reproducible experimental results across assay runs.

Purity: ≥99% by HPLC
CAS Number: 53-84-9
Molecular Formula: C₂₁H₂₇N₇O₁₄P₂
Molecular Weight: 663.43 g/mol
Amino Acid Count: Coenzyme (not a peptide)
Solubility: Soluble in bacteriostatic water; consult the product COA for specific solvent recommendations.
Storage: Store lyophilized at -20°C; protect from light and moisture. Reconstitute with bacteriostatic water; refrigerate reconstituted solution at 2-8°C.

Frequently Asked Questions

What is NAD+?

NAD+, or nicotinamide adenine dinucleotide, is a central redox cofactor involved in a wide range of cellular metabolic reactions. It is supplied as a biochemical reagent for laboratory research only.

Is NAD+ a peptide?

No. Unlike most items in this catalog, NAD+ is a coenzyme, not a peptide. It has no amino acid sequence, which is why it carries a molecular formula but no residue count.

What is the structure of NAD+?

NAD+ is a dinucleotide with the molecular formula C21H27N7O14P2, a molecular weight of 663.43, and CAS number 53-84-9. It consists of nicotinamide and adenine nucleotides joined through a pair of phosphate groups.

What is the difference between NAD+ and NADH?

They are the oxidized and reduced forms of the same molecule. NAD+ accepts electrons to become NADH, and NADH donates them to revert to NAD+. The ratio between the two is a standard readout of cellular redox state.

What is NAD+ used for in research?

Common applications include dehydrogenase activity assays, NAD+/NADH ratio measurement, cellular respiration and glycolysis studies, and sirtuin and PARP enzyme research, where NAD+ serves as the required substrate.

Why do sirtuin and PARP assays require NAD+?

Both enzyme families consume NAD+ as a substrate rather than merely using it as an electron carrier. Sirtuins cleave it during deacetylation and PARPs consume it during poly-ADP-ribosylation, so NAD+ availability directly limits their activity in assay conditions.

What is the NAD+ salvage pathway?

The salvage pathway regenerates NAD+ from nicotinamide rather than synthesizing it from scratch. It is the dominant route of NAD+ production in most cells, and is the pathway targeted in NNMT inhibition studies.

What form is NAD+ supplied in?

NAD+ ships as a lyophilized powder in a sealed vial. Consult the certificate of analysis for the solvent recommendations that apply to your lot.

What purity is NAD+ supplied at?

Purity is 99% or greater as verified by HPLC. Lot-specific results are reported on the certificate of analysis accompanying each vial, and that figure is what our purity guarantee is measured against.

How should NAD+ be stored?

Store the lyophilized vial at -20 degrees Celsius, protected from light and moisture. Once reconstituted with bacteriostatic water, refrigerate the solution at 2-8 degrees Celsius and follow your laboratory's established protocols.

How is the vial shipped?

Orders leave the same business day when placed before 2pm EST, in an insulated mailer with a phase-change pack. Shipping is free on orders over $150.

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